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KMID : 0900920020260020165
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Korean journal of Animal Reproduction
2002 Volume.26 No. 2 p.165 ~ p.171
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Effect of Proline on First Polar Body Formation in Porcine Primary Oocyte
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Oh H.-J.
Lee E.-J.
Yang B.-C.
Chang W.-K.
Kim J.-S.
Lim J.-K.
Yeo Y.-K.
Della-Fera M.A.
Park Y.-S.
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Abstract
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Follicular fluid (FF) contains an oocyte maturation inhibitor with unknown chemical properties. This study was carried out to chemically define the factor(s) inhibiting cumulus cell denudation (CD) and first polar body formation (PBF). Porcine FF (PFF) was extracted with methanol and the extract was serially separated using gel filtration on Superose 12 and Superdex columns. A Superdex fraction was derived with PITC and analyzed with an amino acid analysis column. The results obtained are as follows; PFF had an activity inhibiting both CD and PBF of porcine primary oocytes. Superdex fractions RV2.11 prepared from PFF exhibited an activity inhibiting CD and PBF. By amino acid analysis, the fraction RV2.11 appeared to be proline having the same activity inhibiting CD and PBF. In conclusion, PFF had oocyte maturation inhibitors, of which proline should inhibit CD and PBF.
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KEYWORD
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Oocyte maturation inhibitor, Follicular fluid, Proline
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